Guaranteed train in the event of a strike on weekdays only The actual arrival and departure times in the individual stations and the guaranteed route may be different from what is indicated
| Stop | Arrival | Departure | Tracks |
|---|---|---|---|
| Civitavecchia | 06:13 | 1 | |
| Santa Marinella | 06:19 | 06:20 | 3 |
| Santa Severa | 06:26 | 06:27 | 2 |
| Marina Di Cerveteri | 06:33 | 06:34 | 1 |
| Ladispoli-Cerveteri | 06:39 | 06:40 | 3 |
| Torre in Pietra | 06:46 | 06:47 | 1 |
| Maccarese-Fregene | 06:52 | 06:53 | 2 |
| Roma Aurelia | 07:01 | 07:02 | 2 |
| Roma San Pietro | 07:09 | 07:10 | 4 |
| Roma Trastevere | 07:14 | 07:15 | 1 |
| Roma Ostiense | 07:21 | 07:22 | 1 |
| Roma Tuscolana | 07:26 | 07:27 | 1 |
| Bari Centrale | 18:01 | 1 | |
| Bari torre a mare | 18:09 | 18:10 | 3 |
| Monopoli | 18:25 | 18:26 | 1 |
| Fasano | 18:34 | 18:35 | 3 |
| Cisternino | 18:40 | 18:41 | 3 |
| Ostuni | 18:48 | 18:49 | 2 |
| Carovigno | 18:55 | 18:56 | 2 |
| Brindisi | 19:13 | 19:14 | 2 |
| San Pietro Vernotico | 19:24 | 19:25 | 2 |
| Squinzano | 19:30 | 19:31 | 2 |
| Roma Termini | 07:33 | - | |
| Lecce | 19:42 | - |