Guaranteed train in the event of a strike on weekdays only The actual arrival and departure times in the individual stations and the guaranteed route may be different from what is indicated
| Stop | Arrival | Departure | Tracks |
|---|---|---|---|
| Civitavecchia | 07:18 | 2 TR | |
| Santa Marinella | 07:26 | 07:27 | 3 |
| Santa Severa | 07:33 | 07:34 | 2 |
| Marina Di Cerveteri | 07:40 | 07:41 | 1 |
| Ladispoli-Cerveteri | 07:45 | 07:46 | 3 |
| Torre in Pietra | 07:52 | 07:53 | 1 |
| Maccarese-Fregene | 07:58 | 07:59 | 2 |
| Bari Centrale | 08:01 | 1 | |
| Bari Torre Quetta | 08:05 | 08:06 | 1 |
| Roma Aurelia | 08:08 | 08:09 | 2 |
| Bari torre a mare | 08:11 | 08:12 | 3 |
| Roma San Pietro | 08:15 | 08:16 | 4 |
| Roma Trastevere | 08:20 | 08:21 | 1 |
| Roma Ostiense | 08:27 | 08:28 | 1 |
| Monopoli | 08:27 | 08:28 | 1 |
| Roma Tuscolana | 08:32 | 08:33 | 1 |
| Fasano | 08:36 | 08:37 | 3 |
| Cisternino | 08:42 | 08:43 | 3 |
| Ostuni | 08:51 | 08:52 | 2 |
| Carovigno | 08:57 | 08:58 | 2 |
| Brindisi | 09:13 | 09:14 | 2 |
| San Pietro Vernotico | 09:24 | 09:25 | 2 |
| Squinzano | 09:30 | 09:31 | 2 |
| Roma Termini | 08:40 | - | |
| Lecce | 09:44 | - |